山口大学教務システム - 3273170193-特別研究

開講年度	開講学部等
2025	大学院創成科学研究科（博士前期）
開講学期	曜日時限	授業形態	AL（アクティブ・ラーニング）ポイント
２年通年	卒研・卒論
時間割番号	科目名[英文名]			使用言語	単位数
3273170193	特別研究[Special Research]			日本語	6
担当教員（責任）[ローマ字表記]					メディア授業
執行正義[SHIGYO Masayoshi]					ー
担当教員[ローマ字表記]
執行正義　[SHIGYO Masayoshi]
特定科目区分		対象学生		対象年次	1
ディプロマ・ポリシーに関わる項目			カリキュラムマップ(授業科目とDPとの対応関係はこちらから閲覧できます)

授業の目的と概要

For the master's thesis, students experience and take charge of cutting-edge research in vegetable horticulture under the guidance of faculty members. In the course, students begin by discussing research themes, designing test plans using various vegetables, and conducting various experiments in greenhouses, plant factories, and laboratories. The students will be in charge of managing the growing environment starting from sowing, cultivation management, and harvesting, while collecting data on plant material from each growing environment through sampling surveys. Samples collected will be used to measure dry matter weight, leaf area, and yield, as well as to obtain data on soluble carbohydrate and amino acid content by chemical analysis and gene expression by molecular biological analysis. During the growing period, changes in cell division will be studied and the genes involved in flowering and gametogenesis will be determined by observing the crop. These data will be compiled and summarized in figures and tables after the greenhouse and plant factory surveys are completed. In addition, literature related to the results obtained will be surveyed, and a discussion will be given to the results, considering the position of the results obtained in the thesis research in relation to the research background to date. By summarizing these results in writing, the graduation thesis will be completed.

授業の到達目標


Explain the methods necessary to carry out the research topic Find new findings and problems based on the results of their own experiments Discuss research results Take the initiative in conducting experiments Present research results

授業計画

【全体】

In the master's thesis, the students begin by examining the research theme, design a test plan using various vegetables, and conduct various experiments in greenhouses, plant factories, and laboratories. While being in charge of the management of the growing environment, starting from sowing, cultivation management, and up to harvest, crop data are collected from each growing environment through sampling surveys. Samples collected will be used to measure dry matter weight, leaf area, and yield, as well as to obtain data on soluble carbohydrate and amino acid content by chemical analysis and gene expression by molecular biological analysis. During the growth period, changes in cell division will be studied and plant bodies will be observed to determine the genes involved in flowering and gametogenesis. These data will be compiled and summarized in figures and tables after the greenhouse and plant factory surveys are completed. In addition, literature related to the results obtained will be surveyed, and a discussion will be given to the results, considering the position of the results obtained in the thesis research in relation to the research background to date. These results will be described and published as a graduation thesis in the following order: title, abstract, introduction, materials and methods, results, discussion, and bibliography.

	項目	内容	授業時間外学習	備考

第1回	Research theme planning	Develop a research theme and its objectives.	Reading past theses from the lab (4 hours)

第2回

Research History

An overview of what relevant research has been done leading up to the thesis research.

Organizing a research history from the preface of a previous thesis (4 hours)

第3回

Research Methodology

To study the test methods and component analysis methods in greenhouses and plant factories.

Investigation of experimental methods from laboratory manuals and previous literature (4 hours)

第4回

Test Design

Design the study from the research design method.

Review statistical t-tests, analysis of variance, and principal component analysis. (4 hours)

第5回

Preparation of greenhouses and plant factories

Take actual measurements of test plots in the greenhouse or plant factory to confirm the light environment. Adjust liquid fertilizer for potting and hydroponics as well as activate the irrigation system.

Create a test design and calculate light levels and nitrogen, phosphate, and potassium fertilization rates (4 hours)

第6回

Sowing and establishment of test plots

Check the condition of the growing medium in the test area in the greenhouse/plant factory, prepare the germination beds and sow the seedlings.

Prepare seeds and place in small bags (4 hours)

第7回

Management and maintenance of facilities

After germination of test plants, well-grown seedlings are transplanted to seedling beds.

Create a row planting diagram for individual identification (4 hours)

第8回

Fertilization and pest and weed control

Spraying liquid fertilizer, fungicides and insecticides.

Calculate and prepare the amount of fertilizer to be applied based on nitrogen content. Also, adjust the concentration of the chemical and prepare for spraying (4 hours).

第9回

Observation and investigation of cell division

Sampling of floral organs of plantlets and observational recording of meiosis process using a biological microscope.

Periodic sampling and investigation of plant material. (4 hours)

第10回

Plant leaf color survey

Measure SPAD and color values.

Prepare survey forms for measurement (4 hours)

第11回

Sampling of plant material for genetic analysis

The organs are obtained from individual plants growing in a greenhouse by site, freeze-dried, and then powdered.

Prepare sampling tubes, weigh lyophilized samples, and test for RNA extraction (4 hours)

第12回

Measurement of dry matter weight

The sampled plant samples are air-dried at 70°C for 48 hours, cooled in sealed bags, and weighed for dry matter weight.

Keep the dryer running and under control (4 hours)

第13回

sensory evaluation

Sampling will be conducted from greenhouses and plant factories as appropriate, and sensory evaluations will be conducted by laboratory members.

Prepare sensory evaluation sheets and make preparations to facilitate the test (4 hours)

第14回

yield survey

After fresh weight is measured in a greenhouse or plant factory, each phenotypic value is measured by air-drying after obtaining photographs and 3-D phenotypic models of each individual.

Be prepared to take photographs and 3D. In addition, identify equipment to be used in the preparation of dry samples (4 hours).

第15回

Harvesting and cleaning up test plots

Clean up the greenhouse/plant plant after harvest. Collect all installed equipment and tools and clean growing pots and shelves.

Equipment put away must be organized and stored for use the next year (4 hours)

第16回

Sample Preparation

Dried leaf blade and root samples are crushed for extraction and analysis in chemical analysis.

Pre-dry the sample. Maintain food processor (4 hours).

第17回

Chemical Analysis (Nutritional Composition)

Determine the content of sugars, organic acids, pigments, etc. in plant samples by HPLC method.

Prepare laboratory equipment and adjust reagents. (4 hours)

第18回

Chemical analysis (amino acids)

Determine amino acid content in plant samples by post-column derivatization method.

Prepare laboratory equipment and adjust reagents. (4 hours)

第19回

Chemical analysis (starch)

Determine the starch content in plant samples by colorimetric determination method.

To grind the sample and prepare the sample (4 hours)

第20回

Data Aggregation

Tabulate data using tabulation software Excel

Must be proficient in the use of Excel (4 hours)

第21回

Creating charts and tables

Create charts and diagrams with PowerPoint using Excel's drafting and tabulation functions.

Must be proficient in the use of Excel and PowerPoint (4 hours)

第22回

Statistical processing

Data on Excel is converted and loaded into the statistical analysis software SPSS to perform statistical processing such as t-test, analysis of variance, and principal component analysis.

Learn to use SPSS (4 hours)

第23回

Data Interpretation

Clean up the chart and itemize the results that can be read from it.

Refer to the results of previous theses (4 hours).

第24回

Interim presentation of research results

Acquire presentation skills through monthly “data review meetings” and an interim presentation in December.

For the interim presentation, prepare a draft of the presentation and practice the presentation thoroughly (4 hours).

第25回

Materials and Methods, Writing and Correction of Results

The materials, methods and results will be written. This will then be corrected.

Review seminar science composition techniques (4 hours).

第26回

Survey of related studies

Search and collect literature related to your own research findings.

Students should be able to use article search software at Yamaguchi University Library, the National Diet Library, etc. (4 hours)

第27回

Create a research list

Prepare and list citations for articles related to your own research findings.

Read the paper and prepare a citation (4 hours)

第28回

Writing and revising the introduction and discussion

Write an introduction and discussion based on the list of quotations you have prepared.

Discuss your research findings with your supervisor and friends (4 hours)

第29回

Presentation

He will present his thesis at the Master's Thesis Presentation of the Department of Bioresource and Environmental Sciences in February.

Prepare and draft a presentation and practice presenting it thoroughly (4 hours)

第30回

Submission of Master's thesis

Submit a printed and bound copy of the written master's thesis.

Multiple final checks of the manuscript should be made to ensure that the manuscript is error-free. (4 hours)

※AL（アクティブ・ラーニング）欄に関する注
・授業全体で、AL（アクティブ・ラーニング）が占める時間の割合を、それぞれの項目ごとに示しています。
・A〜Dのアルファベットは、以下の学修形態を指しています。
【A：グループワーク】、【B：ディスカッション・ディベート】、【C：フィールドワーク（実験・実習、演習を含む）】、【D：プレゼンテーション】

A: --%　B: --%　C: --%　D: --%

成績評価法

Evaluation will be based on reports.
Report 100%

教科書にかかわる情報

備考

参考書にかかわる情報

備考

メッセージ

Since vegetables (alliums, spinach, lettuce, squash, etc.) are used as research materials, the students are expected to complete the experiments throughout the year.

キーワード

Vegetables, nutritional ingredients, functional ingredients, plant factories, genome breeding

持続可能な開発目標（SDGs）

(飢餓)飢餓を終わらせ、食料安全保障及び栄養改善を実現し、持続可能な農業を促進する。

(インフラ、産業化、イノベーション)強靱（レジリエント）なインフラ構築、包摂的かつ持続可能な産業化の促進及びイノベーションの推進を図る。

(持続可能な生産と消費)持続可能な生産消費形態を確保する。

メディア授業