山口大学教務システム - 3273170193-特別研究

開講年度	開講学部等
2026	大学院創成科学研究科（博士前期）
開講学期	曜日時限	授業形態	AL（アクティブ・ラーニング）ポイント
２年通年	卒研・卒論		10.0
時間割番号	科目名[英文名]			使用言語	単位数
3273170193	特別研究[Special Research]			日本語	6
担当教員（責任）[ローマ字表記]					メディア授業
執行正義[SHIGYO Masayoshi]					ー
担当教員[ローマ字表記]
執行正義　[SHIGYO Masayoshi]
特定科目区分		対象学生		対象年次	1
ディプロマ・ポリシーに関わる項目			カリキュラムマップ(授業科目とDPとの対応関係はこちらから閲覧できます)

授業の目的と概要

Under faculty guidance, students will systematically experience and conduct cutting-edge research in vegetable horticulture to complete their research plans. Starting with the formulation of research themes, the program covers specific experimental processes including cultivation management in greenhouses and plant factories, sampling, chemical analysis, and molecular biological analysis (such as obtaining gene expression data). Students will statistically analyze and graph the obtained data, add commentary based on existing literature reviews, and cultivate the ability to logically synthesize research findings.

授業の到達目標

1. Can accurately describe the experimental methods and logical frameworks necessary to carry out the research theme.
2. Objectively analyze the results of experiments conducted independently and identify new findings or remaining challenges.
3. Discuss and debate research findings with others based on specialized background knowledge.
4. Develop an attitude of proactively engaging in research (experiments, surveys, management).
5. Effectively present research outcomes using appropriate materials.

授業計画

【全体】

To cultivate the ability to formulate research plans and synthesize research findings, students will systematically experience and execute cutting-edge research in vegetable horticulture under the guidance of an advisor.
First, students learn methods for examining research themes and designing specific experimental plans based on them, then initiate various experiments in greenhouses, plant factories, and laboratories. They personally manage the entire process from sowing to cultivation management and harvesting, learning appropriate methods for controlling the cultivation environment. Through sampling surveys, they collect plant body data related to environmental responses.
For collected samples, students acquire practical techniques including morphological surveys (measuring dry weight, leaf area, yield), chemical analysis using HPLC (quantifying soluble carbohydrates and amino acid content), and molecular biological analysis (acquiring gene expression data). Furthermore, through investigating cell division dynamics during the growth period and detailed plant body observations, they analyze gene functions involved in flowering and gamete formation.
Students will develop skills to statistically aggregate the obtained data after the investigation and compile it into appropriate figures and tables. Furthermore, based on extensive literature review, they will logically consider the positioning of their own findings within the existing research background. By logically synthesizing these outcomes and discussing them with their academic advisor, students will complete the foundational competencies required of researchers and professional practitioners.

	項目	内容	授業時間外学習	備考

第1回	Research theme planning	Develop a research theme and its objectives.	Reading past theses from the lab (4 hours)

第2回

Research History

An overview of what relevant research has been done leading up to the thesis research.

Organizing a research history from the preface of a previous thesis (4 hours)

第3回

Research Methodology

To study the test methods and component analysis methods in greenhouses and plant factories.

Investigation of experimental methods from laboratory manuals and previous literature (4 hours)

第4回

Test Design

Design the study from the research design method.

Review statistical t-tests, analysis of variance, and principal component analysis. (4 hours)

第5回

Preparation of greenhouses and plant factories

Take actual measurements of test plots in the greenhouse or plant factory to confirm the light environment. Adjust liquid fertilizer for potting and hydroponics as well as activate the irrigation system.

Create a test design and calculate light levels and nitrogen, phosphate, and potassium fertilization rates (4 hours)

第6回

Sowing and establishment of test plots

Check the condition of the growing medium in the test area in the greenhouse/plant factory, prepare the germination beds and sow the seedlings.

Prepare seeds and place in small bags (4 hours)

第7回

Management and maintenance of facilities

After germination of test plants, well-grown seedlings are transplanted to seedling beds.

Create a row planting diagram for individual identification (4 hours)

第8回

Fertilization and pest and weed control

Spraying liquid fertilizer, fungicides and insecticides.

Calculate and prepare the amount of fertilizer to be applied based on nitrogen content. Also, adjust the concentration of the chemical and prepare for spraying (4 hours).

第9回

Observation and investigation of cell division

Sampling of floral organs of plantlets and observational recording of meiosis process using a biological microscope.

Periodic sampling and investigation of plant material. (4 hours)

第10回

Plant leaf color survey

Measure SPAD and color values.

Prepare survey forms for measurement (4 hours)

第11回

Sampling of plant material for genetic analysis

The organs are obtained from individual plants growing in a greenhouse by site, freeze-dried, and then powdered.

Prepare sampling tubes, weigh lyophilized samples, and test for RNA extraction (4 hours)

第12回

Measurement of dry matter weight

The sampled plant samples are air-dried at 70°C for 48 hours, cooled in sealed bags, and weighed for dry matter weight.

Keep the dryer running and under control (4 hours)

第13回

sensory evaluation

Sampling will be conducted from greenhouses and plant factories as appropriate, and sensory evaluations will be conducted by laboratory members.

Prepare sensory evaluation sheets and make preparations to facilitate the test (4 hours)

第14回

yield survey

After fresh weight is measured in a greenhouse or plant factory, each phenotypic value is measured by air-drying after obtaining photographs and 3-D phenotypic models of each individual.

Be prepared to take photographs and 3D. In addition, identify equipment to be used in the preparation of dry samples (4 hours).

第15回

Harvesting and cleaning up test plots

Clean up the greenhouse/plant plant after harvest. Collect all installed equipment and tools and clean growing pots and shelves.

Equipment put away must be organized and stored for use the next year (4 hours)

第16回

Sample Preparation

Dried leaf blade and root samples are crushed for extraction and analysis in chemical analysis.

Pre-dry the sample. Maintain food processor (4 hours).

第17回

Chemical Analysis (Nutritional Composition)

Determine the content of sugars, organic acids, pigments, etc. in plant samples by HPLC method.

Prepare laboratory equipment and adjust reagents. (4 hours)

第18回

Chemical analysis (amino acids)

Determine amino acid content in plant samples by post-column derivatization method.

Prepare laboratory equipment and adjust reagents. (4 hours)

第19回

Chemical analysis (starch)

Determine the starch content in plant samples by colorimetric determination method.

To grind the sample and prepare the sample (4 hours)

第20回

Data Aggregation

Tabulate data using tabulation software Excel

Must be proficient in the use of Excel (4 hours)

第21回

Creating charts and tables

Create charts and diagrams with PowerPoint using Excel's drafting and tabulation functions.

Must be proficient in the use of Excel and PowerPoint (4 hours)

第22回

Statistical processing

Data on Excel is converted and loaded into the statistical analysis software SPSS to perform statistical processing such as t-test, analysis of variance, and principal component analysis.

Learn to use SPSS (4 hours)

第23回

Data Interpretation

Clean up the chart and itemize the results that can be read from it.

Refer to the results of previous theses (4 hours).

第24回

Interim presentation of research results

Acquire presentation skills through monthly “data review meetings” and an interim presentation in December.

For the interim presentation, prepare a draft of the presentation and practice the presentation thoroughly (4 hours).

第25回

Materials and Methods, Writing and Correction of Results

The materials, methods and results will be written. This will then be corrected.

Review seminar science composition techniques (4 hours).

第26回

Survey of related studies

Search and collect literature related to your own research findings.

Students should be able to use article search software at Yamaguchi University Library, the National Diet Library, etc. (4 hours)

第27回

Create a research list

Prepare and list citations for articles related to your own research findings.

Read the paper and prepare a citation (4 hours)

第28回

Writing and revising the introduction and discussion

Based on the list of quotations created, consider how to write the introduction and discussion.

Discuss your research findings with your supervisor and friends (4 hours)

第29回

Presentation

Acquire the methods of research presentation.

Prepare and draft a presentation and practice presenting it thoroughly (4 hours)

第30回

Understanding and Verifying the Format of Academic Papers

Understand the format of a paper.

Considering Methods for Verifying Thesis Format (4 hours)

※AL（アクティブ・ラーニング）欄に関する注
・授業全体で、AL（アクティブ・ラーニング）が占める時間の割合を、それぞれの項目ごとに示しています。
・A〜Dのアルファベットは、以下の学修形態を指しています。
【A：グループワーク】、【B：ディスカッション・ディベート】、【C：フィールドワーク（実験・実習、演習を含む）】、【D：プレゼンテーション】

A: --%　B: 10%　C: 80%　D: 10%

成績評価法

Evaluation will be based on reports.
Report 100%

教科書にかかわる情報

備考

参考書にかかわる情報

備考

メッセージ

Since vegetables (alliums, spinach, lettuce, squash, etc.) are used as research materials, the students are expected to complete the experiments throughout the year.

キーワード

Vegetables, nutritional ingredients, functional ingredients, plant factories, genome breeding

持続可能な開発目標（SDGs）

(飢餓)飢餓を終わらせ、食料安全保障及び栄養改善を実現し、持続可能な農業を促進する。

(インフラ、産業化、イノベーション)強靱（レジリエント）なインフラ構築、包摂的かつ持続可能な産業化の促進及びイノベーションの推進を図る。

(持続可能な生産と消費)持続可能な生産消費形態を確保する。

メディア授業