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開講年度 開講学部等
2025 大学院創成科学研究科(博士前期)
開講学期 曜日時限 授業形態 AL(アクティブ・ラーニング)ポイント
通年集中 集中    
時間割番号 科目名[英文名] 使用言語 単位数
3273180028 Bioimaging[Bioimaging] 英語 2
担当教員(責任)[ローマ字表記] メディア授業
今村 博臣
担当教員[ローマ字表記]
今村 博臣
特定科目区分   対象学生   対象年次 1~2
ディプロマ・ポリシーに関わる項目 カリキュラムマップ(授業科目とDPとの対応関係はこちらから閲覧できます)
授業の目的と概要
Bioimaging is a crucial tool in modern biological and medical research, enabling the visualization and analysis of cellular structures, biomolecules, and physiological processes in real time. This course provides an advanced understanding of bioimaging techniques, their principles, applications, and the latest developments in the field. Through lectures and practical sessions, students will gain both theoretical knowledge and hands-on experience with cutting-edge imaging technologies, including fluorescence microscopy, super-resolution imaging, and live-cell imaging.
授業の到達目標
By the end of this course, students should be able to:
1. Understand the fundamental principles of bioimaging techniques and their applications in life sciences.
2. Compare and contrast various imaging modalities, including their advantages, limitations, and appropriate use cases.
3. Analyze and interpret bioimaging data using appropriate software and image analysis tools.
4. Design and execute imaging experiments tailored to specific biological questions.
5. Critically assess recent developments and research in bioimaging.
授業計画
【全体】
In this class, we will first explain the history and principles of microscopy. We will then give a lecture on fluorescence, which is essential for bioimaging, and explain the various fluorescence microscopy methods currently in use, including super-resolution microscopy. After a lecture on image analysis methods, we will also explain luminescence microscopy and electron microscopy.
項目 内容 授業時間外学習 備考
第1回 Introduction to Bioimaging History, Importance, and Applications Preparation and review (more than 2 hours)
第2回 Optical Microscopy Basics Principles of Light Microscopy Preparation and review (more than 2 hours)
第3回 Fluorescence Microscopy (1) Principles of fluorescence Preparation and review (more than 2 hours)
第4回 Fluorescence Microscopy (2) Fluorophores and filters Preparation and review (more than 2 hours)
第5回 Fluorescent Proteins Structure, mechanisms and applications of fluorescent proteins Preparation and review (more than 2 hours)
第6回 Fluorescent Biosensors Principles and applications of biosensors for bioimaging Preparation and review (more than 2 hours)
第7回 Advanced Fluorescence Microscopy Confocal Microscopy and Multiphoton Imaging Preparation and review (more than 2 hours)
第8回 Super-Resolution Microscopy STED, PALM/STORM Techniques Preparation and review (more than 2 hours)
第9回 Live-Cell Imaging Techniques, Considerations, and Applications Preparation and review (more than 2 hours)
第10回 Bioluminescence and Chemiluminescence Imaging Principles and applications of luminescence imaging Preparation and review (more than 2 hours)
第11回 Image Analysis and Quantification Software, Algorithms, and AI-based Approaches Preparation and review (more than 2 hours)
第12回 FCS, FRAP and FRET Techniques for Molecular Interaction Studies Preparation and review (more than 2 hours)
第13回 Electron Microscopy (EM) TEM, SEM, and Cryo-EM Principles and Applications Preparation and review (more than 2 hours)
第14回 Presentation (1) Preparation for presentation of the experimental results Preparation and review (more than 2 hours)
第15回 Presentation (2) Presentation Report assignment (more than 2 hours)
※AL(アクティブ・ラーニング)欄に関する注
・授業全体で、AL(アクティブ・ラーニング)が占める時間の割合を、それぞれの項目ごとに示しています。
・A〜Dのアルファベットは、以下の学修形態を指しています。
【A:グループワーク】、【B:ディスカッション・ディベート】、【C:フィールドワーク(実験・実習、演習を含む)】、【D:プレゼンテーション】
A: --% B: --% C: --% D: --%
成績評価法
Presentation 100%
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履修条件
連絡先
imamura-h@yamaguchi-u.ac.jp
オフィスアワー
14:00-17:00, weekdays

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