タイトル

開講年度 開講学部等
2026 大学院共同獣医学研究科（博士課程）
開講学期 曜日時限 授業形態 AL（アクティブ・ラーニング）ポイント
通年 木5～7 実験・実習 9.0
時間割番号 科目名[英文名] 使用言語 単位数
5083006600 応用獣医学特別実験（栄養代謝学特別実験）[Applied Veterinary Medicine Special Experiment (Nutrition and Matabolism)] 日本語 2
担当教員（責任）[ローマ字表記] メディア授業
渋谷 周作[SHIBUTANI Shusaku] ー
担当教員[ローマ字表記]
渋谷 周作　[SHIBUTANI Shusaku]
特定科目区分   対象学生   対象年次  
ディプロマ・ポリシーに関わる項目 カリキュラムマップ(授業科目とDPとの対応関係はこちらから閲覧できます)
授業の目的と概要
Detection of infectious agents from biological material is performed using genetic diagnostics, quantitative PCR, and immunohistological methods to evaluate immune function in animals, focusing on its relationship to nutrient metabolism, while simultaneously isolating and identifying the infectious agent.
授業の到達目標
To master experimental techniques related to nutritional metabolism and veterinary hygiene.
To conduct experiments on nutritional metabolism and veterinary hygiene while considering their underlying principles.
To develop an interest in understanding nutritional metabolism and veterinary hygiene, and a willingness to apply this knowledge to one's own research.
To think independently about how to advance one's own research and to attempt to apply knowledge from other fields, including this course.
To apply the acquired experimental techniques to one's own research objectives.
授業計画
【全体】
Molecular biology, serology and cellular immunology experiments
項目 内容 授業時間外学習 備考
第1回 Guidance and Experimental Plan Introduce course objectives and outline experiments linking nutrient metabolism, signaling, and viral infection. Preparation on experimental techniques (approx. 2 hours)
第2回 Cell Lines and Culture Conditions Define cell lines and culture media, setting different nutrient conditions (e.g., glucose/AA levels). Preparation on experimental techniques (approx. 2 hours)
第3回 Nutrient Conditions and Cell Growth Assess cell growth and viability under varied nutrient conditions. Preparation on experimental techniques (approx. 2 hours)
第4回 Cellular Signaling 1 Analyze effects of nutrient conditions on mTORC1 signaling by western blotting. Preparation on experimental techniques (approx. 2 hours)
第5回 Cellular Signaling 2 Examine regulation of mTORC1 using amino acid starvation and inhibitors. Preparation on experimental techniques (approx. 2 hours)
第6回 Autophagy Induction 1 Evaluate autophagy induction under starvation using markers like LC3 by western blot. Preparation on experimental techniques (approx. 2 hours)
第7回 Autophagy Induction 2 Visualize autophagic structures using fluorescent probes or tagged LC3. Preparation on experimental techniques (approx. 2 hours)
第8回 Membrane Trafficking Monitor endocytic pathways under different nutrient states. Preparation on experimental techniques (approx. 2 hours)
第9回 Nutrients and Trafficking Compare membrane trafficking dynamics under distinct nutrient conditions. Preparation on experimental techniques (approx. 2 hours)
第10回 Interim Discussion Discuss interim results on nutrient signaling, autophagy, and trafficking. Preparation on experimental techniques (approx. 2 hours)
第11回 Viral Infection Setup Design infection experiments considering virus type, MOI, and safety. Preparation on experimental techniques (approx. 2 hours)
第12回 Nutrients and Infection 1 Evaluate how nutrient conditions affect viral infection efficiency. Preparation on experimental techniques (approx. 2 hours)
第13回 Nutrients and Infection 2 Analyze signaling and autophagy changes in infected cells under different nutrient states. Preparation on experimental techniques (approx. 2 hours)
第14回 Flow Cytometry 1 Use flow cytometry to analyze infection, stress responses, or cell death profiles. Preparation on experimental techniques (approx. 2 hours)
第15回 Flow Cytometry 2 Compare cell populations across combined nutrient and infection conditions. Preparation on experimental techniques (approx. 2 hours)
第16回 Immunofluorescence Visualize subcellular localization of signaling or autophagy‑related proteins. Preparation on experimental techniques (approx. 2 hours)
第17回 Gene Expression 1 Analyze expression changes of selected genes under different nutrient and infection conditions. Preparation on experimental techniques (approx. 2 hours)
第18回 Gene Expression 2 / RNA‑seq Overview Learn how to interpret RNA‑seq/variant data as an extension of expression analysis. Preparation on experimental techniques (approx. 2 hours)
第19回 Signaling Network Integration Integrate signaling and expression data into a network model. Preparation on experimental techniques (approx. 2 hours)
第20回 Data Analysis 1 Organize and visualize experimental data (graphs, figures, tables). Preparation on experimental techniques (approx. 2 hours)
第21回 Data Analysis 2 Analyze nutrient‑dependent and infection‑dependent changes. Preparation on experimental techniques (approx. 2 hours)
第22回 Individual Theme 1 Define a small project connecting this course to each student's research topic. Preparation on experimental techniques (approx. 2 hours)
第23回 Individual Theme 2 Plan experiments using selected techniques tailored to each theme. Preparation on experimental techniques (approx. 2 hours)
第24回 Individual Experiments 1 Perform individual experiments and optimize conditions. Preparation on experimental techniques (approx. 2 hours)
第25回 Individual Experiments 2 Continue experiments and collect sufficient data. Preparation on experimental techniques (approx. 2 hours)
第26回 Interpretation 1 Summarize data and draft preliminary interpretations. Preparation on experimental techniques (approx. 2 hours)
第27回 Interpretation 2 Discuss results in the context of literature on metabolism, signaling, and infection. Preparation on experimental techniques (approx. 2 hours)
第28回 Presentation Preparation Prepare slides and abstracts for final presentations. Preparation on experimental techniques (approx. 2 hours)
第29回 Final Presentation Present experimental results, including individual themes, followed by Q&A. Preparation on experimental techniques (approx. 2 hours)
第30回 Wrap‑up and Perspectives Review the course and discuss applications of the techniques to future research. Preparation on experimental techniques (approx. 2 hours)
※AL（アクティブ・ラーニング）欄に関する注
・授業全体で、AL（アクティブ・ラーニング）が占める時間の割合を、それぞれの項目ごとに示しています。
・A〜Dのアルファベットは、以下の学修形態を指しています。
【A：グループワーク】、【B：ディスカッション・ディベート】、【C：フィールドワーク（実験・実習、演習を含む）】、【D：プレゼンテーション】
A: --%　B: 20%　C: 50%　D: 20%
成績評価法
Report (including presentation) 80%, class attitude and class participation 20%.
教科書にかかわる情報
備考
参考書にかかわる情報
備考
メッセージ
キーワード
持続可能な開発目標（SDGs）

  • 飢餓をゼロに
  • すべての人に健康と福祉を
  • 安全な水とトイレをみんなに
  • つくる責任つかう責任
  • Life on land
(飢餓)飢餓を終わらせ、食料安全保障及び栄養改善を実現し、持続可能な農業を促進する。
(保健)あらゆる年齢のすべての人々の健康的な生活を確保し、福祉を促進する。
(水・衛生)すべての人々の水と衛生の利用可能性と持続可能な管理を確保する。
(持続可能な生産と消費)持続可能な生産消費形態を確保する。
(陸上資源)陸域生態系の保護、回復、持続可能な利用の推進、持続可能な森林の経営、砂漠化への対処、ならびに土地の劣化の阻止・回復及び生物多様性の損失を阻止する。
関連科目
履修条件
連絡先
shushibu@yamaguchi-u.ac.jp
オフィスアワー
Any time

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